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Dr. Barry Eppley

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Surgeon Dr. Barry Eppley

Processing Methods for Injectable Fat Grafting


Fat grafting as a method of both soft tissue reconstruction and aesthetic augmentation has taken on a dominant role in plastic surgery the past decade. Between the wide availability of donor tissue, its relatively easy harvest and subsequent injectability, it is no wonder that autologous fat transplantation has become so popular. But despite its many favorable features, the predictability of the procedure remains…unpredictable. It is felt that what may have the greatest impact on how well fat survives the injection process is how it has been prepared.

Centrifuged Fat Preparation for Fat Injections Dr Barry Eppley IndianapolisIn the March issue of the journal Plastic and Reconstructive Surgery a paper was published on this topic entitled ‘A Comprehensive In Vitro Comparison of Preparation Techniques for Fat Grafting’. The authors harvested fat from the lower stomach region in 14 patients and processed the fat by decantation, centrifugation and membrane tissue filtration. The resultant preparations were examined by electron microscopy and cell viability studies. The number of stem cells present and their character was assessed by cell surface markers and whether they could differentiate into adipose cells.

Adipocyte cell cultures Dr Barry Eppley IndianapolisTheir results showed that neither preparation method caused significant cell damage nor were measurable differences seen in overall cell viability. Neither method of preparation showed a significantly higher number of adipocyte-derived stem cells. The maximal amount of adipocyte concentration by water removal was achieved by membrane-based tissue filtration. In conclusion, while the properties of liposuction-aspirated fat were influenced by the processing method they were not significantly different. Centrifugation and membrane-based filtration are preferred when possible when access to such devices exist.

This is just one of many laboratory studies that have looked at how the preparation process influences the eventual fat injectate. Despite many proponents as well as manufacturer claims of the superiority of one processing method over another, in vitro and clinical evidence has provided no conclusive proof of one best method. This paper continues to show that some processing method is better than none. Given the many variables in the fat grafting process it may also be that the preparation method is not the critical element, or at least as important as we think, in improving fat injection survival.

Dr. Barry Eppley

Indianapolis, Indiana

Dr. Barry EppleyDr. Barry Eppley

Dr. Barry Eppley is an extensively trained plastic and cosmetic surgeon with more than 20 years of surgical experience. He is both a licensed physician and dentist as well as double board-certified in both Plastic and Reconstructive Surgery and Oral and Maxillofacial Surgery. This training allows him to perform the most complex surgical procedures from cosmetic changes to the face and body to craniofacial surgery. Dr. Eppley has made extensive contributions to plastic surgery starting with the development of several advanced surgical techniques. He is a revered author, lecturer and educator in the field of plastic and cosmetic surgery.

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